RNeasy Kits for RNA Purification

RNeasy Kits and QIAwave RNA Kits deliver highly reproducible yields of total RNA from small to large samples. Total RNA can reliably be purified from small numbers of cells, including a single cell, as well as from small amounts of standard tissues (see figures "Reliable RNA isolation from a single cell", "Highly reproducible yields for sensitive applications" and "High-quality total RNA from fine needle aspirates") using the RNeasy Micro Kit. RNA purified using the RNeasy Micro Kit provides maximum sensitivity with precious samples in quantitative gene expression analyses, such as real-time RT-PCR, by efficient on-column digestion of genomic DNA (see figure "Efficient on-column removal of genomic DNA").Total RNA is easily purified from animal or human cells, animal or human tissues, and yeast (see table “Total RNA yields obtained with RNeasy Kits” and figure "RT-PCR of RNA from as few as 100 cells").

The QIAwave RNA Mini Kit gives you highly reproducible yields of total RNA from animal or human cells, animal or human tissues, and yeast (see table “Total RNA yields obtained with RNeasy and QIAwave RNA Kits"). The performance between our QIAwave RNA Mini Kit and RNeasy Mini Kit is identical because the chemistry is the same. We’ve also shown that both kits outperform competitors’ kits (see figure "QIAwave RNA Mini Kit performance").

Total RNA yields obtained with RNeasy and QIAwave RNA Kits

The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits").

Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA from a variety of samples"). Total RNA is easily purified with the RNeasy Maxi Kit from large amounts of starting material including animal or human cells, animal or human tissues, and yeast cells (see table “Total RNA yields obtained with RNeasy Kits”).

The RNeasy 96 system provides fast and reproducible total RNA purification for high-throughput gene expression profiling. The RNA is suitable for sensitive applications such as quantitative, real-time RT-PCR and microarray analysis (see figure "High-quality RNA for sensitive analysis of a low-copy transcript"). Sample sizes range from 10 to 5 x 105 cells (see figure "RT-PCR of RNA from as few as 100 cells"), and high-quality RNA can be purified from large numbers of samples (see figure "Reproducible yields of high-quality RNA"). Individual variations are low throughout the entire purification process; TaqMan® threshold-cycle values are easily achieved at the end of the process with a coefficient of variation (CV) less than 3% using the QuantiTect Probe RT-PCR Kit (see figure "Repeatability of fully automated RNA purification").